Summary
Type: SPE
Application #: 302410
Catalog#: 730002 - SPE Cartridges, Chromabond, C18, 3ml, PP, 200mg, 50/pk
Detection: The solution was placed in a autosampler vials and 3 µl of the solution were
injected into the gas chromatograph. Pentacaine and its internal standard were
methyllated before analysis, stobadin was
Conditions: Column conditioning: by flushing with 2 ml methanol and 1 ml water
Column washing: with 1 ml water, the residual water was displaced from the cartridge under mild pressure
Elution: For the elution of the retained drug, three 1-ml portions followed by one 2-ml portion of methanol or acetonitrile were applied. After the methanolic elution, 1 ml of 5% triethylamine in methanol was used to elute the drugs totally.
Evaporation: Individual portions of the eluate were collected into 3-ml cone vials containing per 1 μl of a suitable internal standard. The solvent was evaporated to dryness at 55°C under nitrogen, 250 μl of ethyl acetate were added to the dry residue and the vials were agitated on a Vortex for 10 sec.
Authors: Marko V Radová K
Source: Journal of Liquid Chromatography 14(9) 1645-1658 (1991)